RESUMO
Important regulatory roles of long non-coding RNAs (lncRNAs) have been recently found, and reported as useful biomarkers in cancer. To identify a potential expression of the new discovered lncRNA (ARA), during promotes cell proliferation, apoptosis inhibit, migration and cell cycle arrest, we firstly evaluate its expression in two cancer tissues (breast cancer and liver cancer) and then compared its variability expression in tumor versus non-tumor samples. Expression profile of ARA lncRNA was evaluated using qRT-PCR in paired tumor and marginal non-tumor samples collected from patients who had been referred to the Shiraz General. After RNA extraction from tissue samples, cDNA synthesis and RT-qPCR method were performed according to the protocols. ARA lncRNA expression level was calculated using 2-ΔΔCt method. Principal-component analysis followed by receiver operating characteristic curve analyses was performed to evaluate the diagnostic potential of selected lncRNA. Our data revealed a significant upregulation (P < 0.001) of ARA in breast and liver tumor tissues, in comparison to same patients non-tumor marginal samples. Also, there was a significant difference between the expression of ARA lncRNA in breast cancer and liver cancer patients (P < 0.05). In conclusion, the results of our study suggest a possible role of ARA lncRNA in proliferation of breast and liver tissues, as well as its potential usefulness as a novel diagnostic biomarker for breast and liver tumors.
Assuntos
Neoplasias da Mama/genética , Neoplasias Hepáticas/genética , RNA Longo não Codificante/genética , Adulto , Idoso , Apoptose/genética , Biomarcadores Tumorais/genética , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células/genética , Feminino , Regulação Neoplásica da Expressão Gênica/genética , Humanos , Fígado/metabolismo , Masculino , Pessoa de Meia-Idade , Análise de Componente Principal/métodos , Prognóstico , RNA Longo não Codificante/fisiologia , Ativação Transcricional , Regulação para CimaRESUMO
OBJECTIVE: Diazinon causes oxidative stress and dysfunction of the liver. This study was undertaken to evaluate the effects of the hydroalcoholic extract of Terminalia chebula, on some biochemical and histopathological parameters of liver tissue in diazinon-administered rats. MATERIALS AND METHODS: Wistar rats were orally administered with 25 mg/kg body weight diazinon. Vehicle (distilled water) and silymarin (50 mg/kg body weight) were used as the negative and positive control groups, respectively. Diazinon-administered groups were treated with T.chebula (Terminalia chebula) fruit extract (200, 400, and 800 mg/kg). After 15 days of treatment, the blood specimens and liver samples were examined. RESULTS: In diazinon-treated group, the levels of serum urea, high density lipoprotein (HDL), and liver superoxide dismutase (SOD), catalase (CAT), and vitamin C significantly decreased (p<0.05) compared to control. Also, in this group, serum triglyceride (TG), total cholesterol (TC), very low density lipoprotein cholesterol (VLDL), protein carbonyl (PC), malondialdehyde, tumor necrosis factor-α (TNF-α), and TNF-α gene expression significantly increased (p<0.05) as compared to the control (vehicle-treated rats). Treatment with T. chebula resulted in a significant increase (p<0.05) in CAT, SOD, vitamin C, HDL and a significant decrease (p<0.05) in the level of urea, MDA, PC, TG, TC, VLDL, TNF-α protein, and the gene expression of TNF-α compared with test without treatment group. Histopathological evidence demonstrated that treatment with T. chebula extract could decrease liver lymphocyte infiltration. CONCLUSION: The present study suggests that T. chebula fruit extract has protective effects against diazinon-induced oxidative stress.
